The Influence of Contrast on Growth of Adipose Mesenchymal Stem Cell Culture
Author : G. R. Soekarno, I. G. R. Suryawan, Andrianto
Upload Date : 19-04-2018
Background : Stem cell therapy in the field of cardiology is increasingly being used. Adipose Mesenchymal Stem Cell is a cell culture that is expected to provide clinical improvement in patients receiving the therapy. One way of stem cell delivery is to use cardiac catheterization assisted by the use of contrast to see where the stenosis is and where the stem cell will be administered. Until now, no studies have looked at the effect of contrast on the proliferation or viability of stem cells. This study was conducted to see how the effect of contrast on the growth of Adipose Mesenchymal Stem Cell culture in vitro.
Objective : To analyze the effect of contrast on the growth of Adipose Mesenchymal Stem Cell
Methods : This research is an experimental post-test control group study. Adipose Mesenchymal Stem Cell (AMSC) samples were obtained from the thawing process at ITD Unair. The samples were then cultured and checked for markers that showed the samples were mesunchymal stem cells immunohistochemically, then divided into 2 groups ie the treated group in the form of contrast iodixanol and the control group. The treatment group was subdivided into 7 subgroups, which received dose of 1 mg / mL, 500 mg / ml, 250 mg / ml, 125 mg / ml, 62.5 mg / ml, 31.25 mg / ml and 15,625 mg / ml, and incubated for 24 hours. AMSC proliferation was assessed using MTT Cell Proliferation Assay. Data analysis was performed by using T 2 free sample test and ANOVA.
Results : MTT Cell Proliferation Assay showed no significant increase or decrease in AMSC proliferation with iodixanol contrast at each dose when compared to the control group.
Conclusion : Contrast of iodixanol has no effect on AMSC proliferation.
KEYWORDS : AMSC proliferation, contrast iodixanol