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Antihypertensive Activity of Oligopeptide Garlic (Allium sativum) Extract in Hypertensive Rat

Author : G. M. Bandong, A. Pratama, B. Bintoro, S. Rahardjo
Upload Date : 19-04-2018

Hypertension is a common health problem that affects many people around the world. Garlic (Allium sativum) has long been used both as a food and traditional medicine. Several studies have shown  potential of garlic (Allium sativum) as an antihypertensive. In previous in vitro study found that dipeptide on garlic extract after hydrolyzed by the enzyme papain have ACE inhibitory activity. These study aims to investigate effect of garlic extracts hydrolyzed in hypertensive rat to decrease of blood pressure.

The study was an in vivo study with Control Group Time Series Experimental Design. Parameter in these study was blood pressure. Before treatment, 30 subjects induced hypertension by 2K1C (Two kidney One Clamp) method. In the sixth week after induction, treatment begun. Treatment lasted for four weeks. The recent study was conducted on 36 Rattus norvegicus, Wistar strain, aged 8-10th weeks were divided into 6 groups: normal / healthy (were not induced), hypertension were given captopril, hypertension were given extracts A.sativum 25mg / kg body weight, hypertension were given A.sativum extract 50mg / kg body weight, hypertension were given extracts A.sativum 75mg / kg, and hypertension without treatment. Blood pressure was measured weekly by tail-cuff method. Data were analyzed by analysis of Linear Mixed Model and significance differences analyzed with Bonferroni test.

Statistical test results showed that there were significant differences in blood pressure between the groups after treatment (p <0.05). Hypertension group (pain control) had a significant difference in blood pressure with healthy group (p = 0.000), hypertension group were given captopril (p = 0.000), hypertension group were given A.sativum dose 50mg / kg body weight (p = 0.013), and 75mg / kg body weight (p = 0.012). Hypertension group given A.sativum 25 mg / kg did not have significant differences in hypertension group (p = 0.051). In the captopril group, test results showed that there were no significant differences in blood pressure with all the group given A.sativum extract (25mg / kg, 50mg / kg and 75mg / kg) (p = 1.000).

Conclusions of the study are significant differences in blood pressure of hypertensive rats fed extracts A.sativum with hypertension rats treated with the most effective dose is 75mg / kg. There was no significant difference in activity between the extract reduced blood pressure with antihypertensive drugs captopril A.sativum (positive control).

KEYWORDS : Allium sativum; hypertension; Angiotensin I-converting enzymes; Papain; Oligopeptides


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