Effects of Garlic Extract (Allicin) on Proliferation of Endothelial Progenitor Cell (EPC) in Stable Coronary Artery Disease Patients

Author : A.Y. Putri, B.S. Pikir, Y.H. Oktaviono
Upload Date : 19-04-2018

Background : Endothelial Progenitor Cell (EPC) has a role to the atherosclerotic process that leads into pathogenesis of coronary artery disease. The reduced number and function of EPC in stable coronary artery disease (SCAD) patients will further aggravate endothelial dysfunction and inhibit neovascularization. Garlic with all its benefit in cardiovascular is currently believed to increase the number and function of EPC. Therefore, this in vitro study was conducted to analyze the effect of garlic extract (Allicin) on the proliferation of EPC. The aim of this study is to analyze the effect of garlic extract (Allicin) on EPC proliferation in patients with SCAD

Methods : This study is a quasi-experimental post-test control group study. The mononuclear cells were isolated from peripheral blood of SCAD patients and cultured on CFU-Hill medium for three days. The samples were then divided into 2 groups, treatment with garlic extract (Allicin) group and control group. The treatment group was then subdivided into 3 subgroups, which received 10 mg/ml, 50 mg/ml, dan 100 mg/ml of doses and incubated for 48 hours. EPC proliferation was assessed using MTT Cell Proliferation Assay. Immunohistochemical method of CD34+ were performed to EPC identification. Evaluation on CFU-Hill was done to confirm the functional characteristic of EPC. Data was analyzed by using independent T test and ANOVA

Results : MTT Cell Proliferation Assay showed a significant increase in EPC proliferation by addition of garlic extract (Allicin) compared with control group (0.2811 ± 0.008 vs 0.194 ± 0.151 p<0.05) and a significant improvement were also observed in each dose increment. CFU-Hill quantification shows the addition of EPC colony in high-dose of Allicin. Immunohistochemical method shows positive CD34+ expression

Conclusion : Garlic extract (Allicin) increases EPC proliferation dose-dependently from peripheral blood of SCAD patients

KEYWORDS : EPC proliferation, SCAD, Allicin

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