Association between Diabetes Mellitus and Micro-RNA34a Expression in Autologous Bone Marrow Stem Cell in Patients with Coronary Heart Disease Undergoing Stem Cell Therapy

Author : M.G.F. Harmani, R. Sukmawan, B. Widyantoro
Upload Date : 19-04-2018

Background: Bone marrow stem cell (BMSC) therapy is one of the developing options in regenerative therapy for patients with CHD. It is important to recognize factors that will affect the stem cell quality, especially its survivability, to improve clinical outcomes after stem cell therapy. Increased expression of miR-34a will induce more apoptosis in BMSC. Diabetes mellitus (DM) has been known to increase miR-34a expression in aortic endothelial lining and serum. But to this day, no study has evaluated the association between diabetes mellitus and miR-34a expression in BMSC.

Objective: To evaluate the relationship between DM and miR-34a expression in BMSC and to evaluate correlation between HbA1C and miR-34a expression in BMSC in CHD patients who underwent bone marrow stem cell therapy.

Methods: This is a cross sectional study which included all CHD patients undergoing stem cell therapy in National Cardiovascular Center Harapan Kita (NCCHK). Primary data are miR-34 expression in bone marrow stem cell taken from subject’s bone marrow aspiration, while secondary data were taken from medical records. MiR-34a analysis was carried out using the Taqman fast mastermix 7500 assay with real time PCR.

Results: There were 24 CHD patients undergoing stem cell therapy. Two group were compared, DM with 13 patients and non DM with 11 patients. Examination of HbA1c was done only in the DM group with mean value was 7.3+1.5. There seems to be an increase in miR-34a expression in patients with DM (0,3+0,24 vs 0,05+0,08, p = 0.9). There is a positive correlation between HbA1c in DM population and miR-34a expression (r=0.601 and p=0.039).

Conclusion There is a non significant trend between diabetes mellitus and miR-34a expression in BMSC in CHD patients. In CHD patients with diabetes mellitus patients, HbA1C correlates positively with miR-34a expression. The higher HbA1C, the higher miR-34a expression in BMSC.

KEYWORDS : diabetes mellitus, miR-34a, bone marrow stem cell, HbA1c

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