Hypoxia Preconditioning Effect on the Expression of Intracelullar Reactive Oxygen Species (ROS) Levels on Cultured Adipose Mesenchymal Stem Cells
Author : R. D. Cahyaningtias, R. Suryawan, Andrianto
Upload Date : 19-04-2018
Background: We studied the stimulation effect of Hypoxia Preconditioning in ASCs cultured on expression of Reactive Oxygen Species (ROS) and Superoxide Dismutase (SOD) intracellular. The survival of transplanted stem cells is reported to be low and still became a main problem in regenerative medicine. Hypoxia Preconditioning (HPC) is a sublethal hypoxia that stimulates AMSCs to a defense mechanism to protect stem cell from lethal hypoxia and ischemic condition. This HPC may can induced spesific ROS generation from NADPH Oxidase enzyme (NOX) Family in cytosolic membrane and mitochondria which can play pivotal role as second messengers and activated normal cellular processes especially in redox signalling. ROS as an double swedge sword that in low levels of ROS as a friend not a foe.
Methods: This study using In-Vitro True Experimental with Randomised Controlled Post Test Design in human AMSCs. Isolated human AMSCs from minimaly invasive and then cultured until passage fourth. Identification AMSCs with expression of CD 105, CD 90 and CD 45. This sample divided into controlled group (Normoxia 21% O2) and Hypoksia group (HPC 1% O2). Expression of intracellular ROS and intracellular SOD was evaluated with immunofluorescence methods.
Results: Expression of ROS is lower in hypoxia group than in controlled group (253.13 ± 67.795 vs 342.13± 116.447 p < 0.05) and SOD expression is higher in hypoxia group than in controlled group (340.25 ± 96.476 vs 234.56± 38.238 p <0.05).
Conclusion: Hypoxia Preconditioning with 1% O2 in human AMSCs gives a lower expression of intracellular ROS and higher expression of intracellular SOD.
KEYWORDS : Hypoxia preconditioning, Redox signalling, Intracellular ROS, Intracellular SOD, Adipose Mesenchymal Stem cell